Biological assays available
Different assays are available to assess the therapeutic potential of your molecules of interest.
Endocrinology
Oncology
Different assays are available to assess the therapeutic potential of your molecules of interest.
Endocrinology
- Determination of the inhibition activity of your molecules for different steroidogenesis enzymes: steroid sulfatase,17β-HSD1, 17β-HSD2, 17β-HSD3, 17β-HSD5, 17β-HSD7, 17β-HSD10 (Donald Poirier)
- Determination of the estrogenic, anti-estrogenic, androgenic and antidrogenic activities in vitro (ER+ and AR+ cells) (Donald Poirier)
Oncology
- Test of cell viability (normal and cancer cells*)
(ex: MCF-10A, Lymphocyte, MCF-7*, T-47D*, ZR-75-1*, LNCaP*, PANC-1*, OVCAR-3*, HL-60*, Shionogi*) (Donald Poirier) - Evaluation of the antiproliferative activity of drugs on several tumour cell lines (wild-type or chemoresistant) (René C.-Gaudreault)
- Effect and evaluation of drugs on the cell cycle progression (René C.-Gaudreault)
- Evaluation of the affinity of antimitotics to the colchicine-binding site on tubulins (René C.-Gaudreault)
- Separation and detection of proteins by polyacrylamide gel electrophoresis and western blot (René C.-Gaudreault)
- Evaluation of the antitumor activity and the systemic toxicity of drugs on Human fibrosarcoma tumours (HT-1080) grafted onto the chick chorioallantoic membrane (CAM assay). The assay allows the evaluation of the toxicity of a drug on a developing chick embryo together with its effect on tumour growth and angiogenesis. (René C.-Gaudreault)